Giovanni's library, which was preserved along with the rest of the Bibliotheca Malatestiana, may be the earliest physician's library to have survived intact. The library contains numerous spectacular codices of the expected standard European and Arab scientific and medical authorities, several dating from the 11th, 12th, and 13th centuries, and one (S. XXI.5) dating from the 8th century. Some are finely illuminated. That Giovanni owned several manuscripts from prior centuries suggests that he collected books not only for reference but also out of humanistic and antiquarian interest.
An excellent annotated catalogue of this library was published in large 4to format: Manfron (ed.) La Biblioteca di un Medico del Quattrocento. I codici di Giovanni di Marco da Rimini nella Bibliotheca Malatestiana (1998). The catalogue contains numerous fine color plates.